| Protein name |
Collagen |
| Host compatibility |
Escherichia coli (Guo et al., 2010) |
| Yield |
10.8 g/L (Guo et al., 2010)
|
| Scale (in specific example) |
Pilot scale (Guo et al., 2010)
|
| Biggest scale product has been develloped in this organism |
Pilot scale for food purposes, however in biomedical context, there are companies producing collagen on industrial level in E.coli. |
| Regulatory status in Europe |
Not allowed |
| Regulatory status in other parts of the world |
Collagen production by E.coli K-12 S9188 has FDA GRAS approval in the US
|
| Companies producing this protein in this organism |
|
| Extra/remark |
|
| Publications/references |
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Guo, J., Luo, Y., Fan, D., Yang, B., Gao, P., Ma, X., & Zhu, C. (2010). Medium optimization based on the metabolic‐flux spectrum of recombinant Escherichia coli for high expression of human‐like collagen II. Biotechnology and Applied Biochemistry, 57(2), 55–62. https://doi.org/10.1042/ba20100081
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Gómez-Guillén, M., Giménez, B., López-Caballero, M., & Montero, M. (2011). Functional and bioactive properties of collagen and gelatin from alternative sources: A review. Food Hydrocolloids, 25(8), 1813–1827. https://doi.org/10.1016/j.foodhyd.2011.02.007
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Ahmad, M. I., Li, Y., Pan, J., Liu, F., Dai, H., Fu, Y., Huang, T., Farooq, S., & Zhang, H. (2024). Collagen and gelatin: Structure, properties, and applications in food industry. International Journal of Biological Macromolecules, 254, 128037. https://doi.org/10.1016/j.ijbiomac.2023.128037
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Tang, C., Zhou, K., Zhu, Y., Zhang, W., Xie, Y., Wang, Z., Zhou, H., Yang, T., Zhang, Q., & Xu, B. (2022). Collagen and its derivatives: From structure and properties to their applications in food industry. Food Hydrocolloids, 131, 107748. https://doi.org/10.1016/j.foodhyd.2022.107748
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Fertala, A. (2020). Three Decades of Research on Recombinant Collagens: Reinventing the Wheel or Developing New Biomedical Products? Bioengineering, 7(4), 155. https://doi.org/10.3390/bioengineering7040155
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Browne, S., Zeugolis, D. I., & Pandit, A. (2013). Collagen: Finding a Solution for the Source. Tissue Engineering Part A, 19(13–14), 1491–1494. https://doi.org/10.1089/ten.tea.2012.0721
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Guo, X., Ma, Y., Wang, H., Yin, H., Shi, X., Chen, Y., Gao, G., Sun, L., Wang, J., Wang, Y., & Fan, D. (2023). Status and developmental trends in recombinant collagen preparation technology. Regenerative Biomaterials, 11. https://doi.org/10.1093/rb/rbad106
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